8/1/2023 0 Comments Christina haak nude![]() This construct regulates toxin expression both at the transcriptional level by the use of a chimeric-modified enhancer/promoter sequence of the human prostate-specific antigen gene and by DNA recombination mediated by Flp recombinase. Finally, we used C32 to deliver a DNA construct encoding the A chain of diphtheria toxin (DT-A) to xenografts derived from LNCaP human prostate cancer cells. injection, whereas C32 demonstrated no toxicity. Additionally, polyethyleneimine induced significant local toxicity after i.m. administration were achieved with naked DNA, followed by polyethyleneimine transfection was rarely observed with C32. Conversely, the highest transfection levels after i.m. C32 delivered DNA intratumorally 4-fold better than one of the best commercially available reagents, jetPEI (polyethyleneimine), and 26-fold better than naked DNA. We tested the best performing polymer, C32, in mice for toxicity and DNA delivery after intratumor and i.m. Using high-throughput methods, we screened this library in vitro for transfection efficiency and cytotoxicity. ![]() The HGF-treated mice showed reduced colonic mucosal damage and increased body weights, compared with control mice (P 500 degradable, poly(-amino esters) for potential use as nonviral DNA vectors. Expression of the transgene was seen in surface epithelium, lamina propria, and muscularis mucosae. To explore the therapeutic effects of naked gene therapy of hepatocyte growth factor (HGF) on UC, the SR promoter driving HGF gene was intrarectally administered to the mice in which colitis was induced by dextran sulfate sodium (DSS). ![]() Ulcerative colitis (UC) is progressive and relapsing disease. Kanbe, Takamasa Murai, Rie Mukoyama, Tomoyuki Naked gene therapy of hepatocyte growth factor for dextran sulfate sodium-induced colitis in miceĭOE Office of Scientific and Technical Information (OSTI.GOV) ![]()
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